Lubna Alhudhali

3 Questions 3 Answers 0 Followers

Questions related from Lubna Alhudhali

How to eliminate ghost band in Urea polyacrylamide gel ?

I performed in vitro DNA replication. and run the product on 15 % urea polyacrylamide gel after 5min denaturation in 95 oC. I have been doing it for several times and works fine, however, recently...

09 July 2023 3,395 0 View

How to get rid of the host proteins (E.coli) during my protein purification ?

am trying to purify a human DNA binding protein (normally localized in nucleus and mitochondria). I am currently optimizing the conditions I tried 2 different approaches: 1. HisTrap-> TEV -->...

06 December 2022 5,032 5 View

Can you please help me, to know why I have intense band in my SDS page at pH9?

let me first explain my SDS gel, the left 4 lanes ( Lane 1 to 4 ) contain individuals proteins. the right 4 lanes (lane 5 to 8) contain a mixture of the individual proteins with different buffers....

31 January 2022 1,317 0 View