Louise M Burmeister

3 Questions 1 Answers 0 Followers

Questions related from Louise M Burmeister

Can the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina be used for sequencing of PCR amplicons?

We have around 80 PCR amplicons, amplified in 3 multiplex pools, that we wish to sequence on a MiSeq. Our products are all between 200-300 bp in size, and we were planning to use the NEBNext®...

04 December 2019 5,435 1 View

Would very short reads impact the appearance of a compound heterozygote?

I have recently sequenced a number of PCR amplicons on an Illumina MiSeq, in a sample that is a compund heterozygote for 2 SNPs. The 2 SNPs are very close together, so in theory any read that...

08 May 2019 5,069 0 View

Can protein expression be altered even if mRNA expression is not?

I am investigating a nonsense SNP mutation which is predicted to result in a protein truncated from 680 to less than 300 amino acids. We think this causes a relatively severe disease. As far as...

20 September 2018 5,454 1 View