Louis Suarez

4 Questions 4 Answers 0 Followers

Questions related from Louis Suarez

Can lysozyme also interact with dextran and agarose?

Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...

26 May 2024 5,914 3 View

What is the reason for quickly removing imidazole after purifying a protein?

I was told by a lab colleague that 'after purifying a protein with a his-tag using imidazole, it is crucial to quickly remove the imidazole. Keeping the protein in more than 200mM of imidazole is...

05 March 2024 4,873 1 View

His tag aggregates?

I'm working on isolating recombinant protein in E.coli (Ni-NTA) I'am trying to purify recombinant protein that I overexpressed E.coli BL21(DE3)pLysS using the pCold1 vector. The proteins have...

04 November 2023 3,043 3 View

What is role of the magnesium acetate in cell lysate?

I'm working on preparation of protein lysates from E.coli I read in a few paper that magnesium acetate should be added before sonication what is role of the Mg ace in cell lysis?

04 November 2023 4,831 2 View