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Questions related from Louis Suarez
We measure the relaxation rates of our protein backbone, including a 1H–15N hetero NOE experiment. In my protein, there is a specific region that forms a helix (confirmed by Cα and CO chemical...
22 March 2025 5,764 0 View
Hello everyone, Could someone please teach me the basics of operating an NMR spectrometer using TopSpin? So far, the only experiments I've performed are measuring HSQC and HNCACB spectra. 1. I...
21 November 2024 6,384 3 View
Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...
26 May 2024 6,151 3 View
I was told by a lab colleague that 'after purifying a protein with a his-tag using imidazole, it is crucial to quickly remove the imidazole. Keeping the protein in more than 200mM of imidazole is...
05 March 2024 4,937 1 View
I attempted to dissolve 20 mM DTNB(5,5 - Dithiobis(2-nitrobenzoic acid) in 1X PBS buffer(0.1M pbs, pH 7.3), but it was unsuccessful. Even after adding DTNB to the PBS buffer and vortexing for...
07 February 2024 1,762 4 View
I'm working on isolating recombinant protein in E.coli (Ni-NTA) I'am trying to purify recombinant protein that I overexpressed E.coli BL21(DE3)pLysS using the pCold1 vector. The proteins have...
04 November 2023 3,112 3 View
I'm working on preparation of protein lysates from E.coli I read in a few paper that magnesium acetate should be added before sonication what is role of the Mg ace in cell lysis?
04 November 2023 4,930 2 View
Hello, everyone. My question is basically the same as the title above. Are there instances of intracellular acidification resulting in an extremely low pH (excluding lysosomes)? In 2023, Wei Li...
01 January 1970 7,458 1 View
