Lorraine Quinn

2 Questions 1 Answers 0 Followers

Questions related from Lorraine Quinn

Has anyone carried out an activity assay on pyruvate decarboxylase (PDC) enzyme?

Hi, I'm attempting to carry out an activity assay on a PDC enzyme using an indirect enzyme coupled assay. The reaction is started when PDC in cell extract (which has been heated to 60 degrees...

19 February 2015 8,886 2 View

Hi all, could anyone give me some advice on transformation via electroporation?

I'm currently trying to transform top 10 E.coli cells that have streptomycin resistance with a ~5kb pET22b+ vector with ampicillin resistance in which I have ligated a ~1.7kb product but so far I...

24 November 2014 3,559 3 View