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Why did I get different restriction digestion results from three plasmid preparations, even though they all came from the same single colony of BL21(DE3) carrying the pET-SUMO-Lcp3 plasmid? I...
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mobile phase: methanol/ACN (90:10, v/v) + 9 μM TEA. Can I use n-hexane as the injection solvent? Will this cause column clogging or damage? I know that acetonitrile and n-hexane are immiscible.
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