Lorenzo Gallicchio

4 Questions 5 Answers 0 Followers

Questions related from Lorenzo Gallicchio

Q5 site directed mutagenesis. Many colonies but almost all only contain ampicillin alone. What is happening?

HI, i am encountering some issues with the Q5 site directed mutagenesis kit from NEB. I am supposed to do something very simple in principle which is delete a region of ~100 nt from my template...

01 July 2023 7,481 0 View

How to track RNA/DNA fragments of specific lengths on PAGE gels?

Hi all, I am trying to purify from PAGE gels (with or without UREA) RNA/DNA fragments which lengths are between (for instance) 40 nt and 60 nt. What I though was to produce some sort of...

11 January 2022 2,673 2 View

SmFISH followed by IF, lost signal?

Hi all :) I am trying to perform smFISH followed by immunostaining on drosophila testes but when I check how the staining looks I have no signal from the smFISH probes (while the antibody is...

30 July 2021 4,600 6 View

Blunt cloning using Klenow fragment, what is going wrong?

Hi everyone, I am currently trying to clone a fragment (~1700bp) cut with ECORI into a vector (~5300 bp) linearized by ECORV. Since ECORI leaves cohesive ends I am using Klenow Fragment...

08 December 2018 10,087 5 View