Lorcan Rooney

3 Questions 5 Answers 0 Followers

Questions related from Lorcan Rooney

How can I get better fluorescence sprectra if the Raman Scattering peak of my solvent is higher than that of the fluorescent wells?

I'm performing a serial dilution of Alizarin Red S and a boronic acid. Individually they are not fluorescent, but once complexed, the resulting solution is fluorescent. Despite having issues with...

19 February 2024 3,597 5 View

Why is my acid-polyethyleneimine conjugate precipitate insoluble in water?

In the past I have conjugated an acid to polyethyleneimine with percentage conjugation of around 25%. I wanted to increase this percentage, and so I doubled the amount of acid, EDC and NHS before...

15 May 2023 3,254 2 View

Troubleshooting using Fluorometer for a Beginner - How do I stop getting the same results across all wells in 96 well plate?

I've recently started using an Edinburgh Systems FS5 fluorometer with no experience or training other than the instructional videos they provide on a memory pen. I'd like to know what the best...

24 October 2022 3,949 3 View