What is wrong with discrepancy of sequencing results between gDNA and cDNA after CRISPR/Cas9 gene editing?

Hi everyone, I am trying to use CRISPR/Cas9 to mutate one gene in leukemia cell lines by Lipo3000 (from AGC to AAC). After transfection and puromycin selection of 5 days, the GFP+ of the cells is...

01 January 2020 7,070 3 View