Loka Raghu Kumar Penke

3 Questions 8 Answers 0 Followers

Questions related from Loka Raghu Kumar Penke

How to measure 20S proteasome activity (precisely) from lung tissue?

Hello everyone, My experiment involves administration of different doses of proteasome inhibitors (via i.p.) to mice and harvest lungs to measure the proteasome activity. Before I actually do the...

09 January 2020 6,594 0 View

How to create (a desired promoter driven) GFP reporter vector?

My requirement is GFP reporter to measure the promoter activity qualitatively (instead of lucifrease based quantitative measurement). So, I would like to clone a GFP reporter construct with my...

08 January 2016 571 4 View

Can anyone help with expressing a mammalian protein in bacteria?

I wish to clone a gene (mammalian gene) in a plasmid and express it in bacteria. I need the features for the cloned protein: 1. His tagged (for purification purpose) 2. GFP (to visualize) 3....

23 June 2014 5,983 1 View