What is the role of chloroform, isopropanol, as well as NP-40 during the extraction of cholesterol from lysed cells?

I want to determine whether the centrifugation, separation of organic phase, & chloroform: Isopropanol: NP-40 steps are necessary in sample prep as per cell biolabs Cholesterol Assay (using...

01 January 1970 5,832 3 View