How to calculate mass of dry Tris base + Tris HCl to dissolve to give a buffer with desired concentration?

I am trying to make a 1M Tris Cl pH8 buffer with dry Tris base and Tris HCl. I know that it is possible to make Tris buffers without the use of HCl by combining powdered Tris acid with Tris base....

03 April 2017 775 4 View