Liz J Valente

4 Questions 1 Answers 0 Followers

Questions related from Liz J Valente

Advice on single cell cloning HEK-293F cells?

Can anyone point me to some good guides related to single cell cloning techniques for suspension cell lines, bonus points for guides specific to HEK-293F. Thanks, Liz

20 August 2024 4,121 0 View

Protocol of pellet mammalian cells from a large volume e.g. 500 mL at 4˚C?

Hi, Looking for a protocol to get efficient pelleting of HEK-293 cells from a large volume of media (400-500 mL) in 500 mL conical bottles. Anyone have recommendation for g force and length of...

11 August 2023 8,177 0 View

Favorite cell counters?

My lab is looking to purchase a cell counter for tissue culture and I was hoping you guys might have recommendations for ones that you loved (or hated)? At a minimum we’d need one capable of...

12 February 2023 6,921 2 View

Resources for mammalian cell culture scale up (>200E6 cells)?

Hi! I was hoping someone might be able to recommend some resources relating to scaling up mammalian cell culture, in the range of 200-500E6 cells (either adherent or suspension). I'd be...

14 February 2022 5,272 1 View