Lixin Xiao

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Questions related from Lixin Xiao

Is it normal that K562 cells' sizes vary after viral infection and drug screening？Can K562 cells differntiate to other-shaped cells?

I found two problems when culturing K562 cells. One is that cell size varies after viral infection and Puro(or Blast) screening. The other one is that K562 cells occasionally differentiate to...

16 November 2023 9,964 1 View

Plasmid in Stbl3(competent cell) disappeared after being stored at -80°C, is that -80 making plasmid disappear?

I transformed a library into stbl3 competent cells following a standard protocol which is like incubation 30min in ice, 42°C for 45s, soc culture at 37°C for 1h, before culturing at 30°C on LB...

02 September 2023 9,975 5 View