Liu Yuanhui

2 Questions 9 Answers 0 Followers

Questions related from Liu Yuanhui

Do you know about the siRNA knockdown efficency 30%, how can i make it better?

I transfected the clathrin siRNA at 20nM and 50nM for 2 to 6 days, then lysed the cells, then did the western blot. The image is in the attached annex. The best kncokdown efficiency I can reached...

02 February 2016 4,301 9 View

Why EGFR is observed by IF to be diffused everywhere in the Hep3B cells, while theoretically it is membrane protein?

Dear all, I did the immunofluorescence for Hep3B cells. Cells were fixed by 4% paraformaldehyde 10min and permeabilized by 0.1% TRITON-X100 10min, blocked by 1% BSA 30min and incubated by...

08 August 2015 8,945 5 View