Liu Zhi Fei

2 Questions 3 Answers 0 Followers

Questions related from Liu Zhi Fei

How to make PCR fusion reach the target fragment length?

Hello researchers! Now one of my work is assembling two DNA fragments with homologous arm into one fragments which as 2900 bp length. But according to the results of the PCR, the length of the...

28 December 2021 6,736 7 View

How to efficiently electroprorate Escherichia coli cells

Hello everyone! Recently, I was confused by E.coli electroproration. My goal is to transform the recombinant fragments and pGRB-SgRNA plasmids into competent cells containing pRED-Cas9 by...

01 January 1970 2,957 1 View