Performing IF on fetal liver frozen sections for preHSC/HSC?

I have frozen sections of 4%PFA drop-fixed, 30% sucrose dehydrated fetal livers from E14.5 mouse embryos. I sectioned them both at 10 and 5 microns. When I performed the staining on the 10 micron...

26 June 2020 4,242 1 View