Lisa McDougall

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Questions related from Lisa McDougall

Sanger Interference - Elevated A Signal (Green)?

Has anyone seen anything like this before during Sanger sequencing? We have a control sample that was run inline, and it was perfect, indicating the Sanger setup and instrument were performing...

14 November 2023 7,289 3 View

Questions on Upgrading from an AB 3130xl to an AB 3500 Genetic Analyzer?

We are looking into upgrading to the 8-capillary AB 3500 Genetic Analyzer. We currently have a 16-cap 3130xl, but it's getting discontinued. We are a small core facility that sequences various...

08 December 2021 2,945 1 View

What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

15 January 2021 7,525 6 View