Lisa Kelly

2 Questions 2 Answers 0 Followers

Questions related from Lisa Kelly

Microbiome samples that didn't reach a plateau on rarefaction curve?

I want to compare alpha/beta diversity of some microbiome samples, however, 2/12 samples didn't plateau when I did my rarefaction curves. This tells me that the sequencing depth wasn't sufficient....

03 December 2020 808 3 View

Can you run an nmds ordination on microbiome abundance data or must you normalize the data?

Do you have to normalize microbiome abundance data prior to running an NMDS ordination? I am getting very confused. In the data processing stage, I subsampled the OTU table to a certain read count...

03 December 2020 166 0 View