Does anyone have a protocol to do T7 transcription primed with GMP to introduce an unlabeled 5' phosphate?

I will do RNA ligation by using two fragments. 5' fragment is ordered from Dharmacon; 3' fragment will be transcribled by T7 polymerase. For the 3' fragment, there are two method to treat with...

14 September 2012 8,647 1 View