Lindsey A. Perry

2 Questions 7 Answers 0 Followers

Questions related from Lindsey A. Perry

To clone or not to clone?

I am about to start cloning an archaeal gene into an E. coli vector to use as a standard during qPCR. I am either going to use pGEM - T easy or TOPO A cloning kit. I have not done this before so...

14 April 2015 4,979 4 View

Running a genomic DNA quality check on a gel - can anyone help?

I have extracted gDNA from rumen fluid using a CTAB bead beating method. According to the Nanodrop I have somewhere between 250 and 800ng/ microlitre of good quality (260/280 = 1.8 - 1.9) DNA in...

18 June 2013 6,369 42 View