Lindsey Parker

2 Questions 10 Answers 0 Followers

Questions related from Lindsey Parker

Has anyone noticed poor agreement between replicates using the qiagen one-step RT-PCR kit?

I have been using the kit above for some time now, which performs cDNA conversion and normal qPCR in the same tube. I am noticing that despite using recently calibrated pipettes and preparing my...

01 May 2015 5,114 10 View

What should I do with my primer optimization?

I am optimizing a 16s primer pair and cannot get rid of primer dimers/ nonspecific binding, despite them apparently having no capacity to form primer dimers according to oligoevaluator. They were...

28 June 2014 989 9 View