Linda Ratjama

4 Questions 3 Answers 0 Followers

Questions related from Linda Ratjama

What would be the reasons for inconsistent cytotoxic response of nicotine?

I have a panel of 4 cells for which I am investigating the nicotine dose response curve. For C33a, HeLa and HaCaT cells, the cytotoxicity is dose and time dependent. However, for tonsillar cells,...

06 October 2024 8,309 0 View

Why is my DNA ladder starting halfway in the gel?

I am using a 50bp DNA ladder. I have amplified p53 which was supposed to show at 166bp but is apparently at 500bp according to the image. I think the ladder is the problem, why is it starting so...

23 August 2023 5,929 3 View

Can You help me with a protocol for protein extraction?

I have laryngeal cancer samples from which I am to extract DNA/RNA and Protein. However, the kit I have only extracts DNA and RNA (Qiagen AllPrep DNA/RNA Kit), and does not extract protein. How do...

29 May 2023 9,936 6 View

Can someone please help me interpret my results?

I have been failing to get amplification of PCR and decided to add different concentrations of MgCl2 to my mastermix and the prepared different PCR tubes with their negative controls. The first...

25 May 2023 9,456 4 View