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Questions related from Lincoln Shane
I am currently trying to express human tyrosinase in E.coli and as you know this enzyme being a membrane protein it comes with a fair share of difficulties relating to expression in BL21(DE3)....
04 October 2018 7,119 3 View
I have been trying to express in BL21(DE3), purify and determine the Enzymatic kinetics of human Tyrosinase. After futile attempts at determining the kinetics of my purified protein ( expressed at...
07 May 2018 8,053 9 View
Hello. I have been struggling with purification of human Tyrosinase expressed in E. Coli. In brief, I induce expression in E, Coli BL21 at an OD of 0.6 and then incubate further for 3 hours at 37...
07 May 2018 3,324 2 View
Hello fellow scientists, here i have a bit of a seemingly demoralizing issue with protein purification. Could you please help? I have attached a commassie image for clarity. 10mM imidazole, 50 mM...
26 December 2017 3,971 4 View
Hello everyone out there. I am doing an epidemiological study of clostridium difficile. I am using eBURST program to assign MLST STs to clonal groups or complexes. The program has assigned them to...
19 May 2016 845 0 View
Hello fellow researchers, I am having a bit of an issue. I have been trying to extract DNA from skimmed milk clinical isolates in vain. The extraction is as follows: Add BHI plate cultured...
22 October 2015 8,720 6 View