Lincoln Shane

6 Questions 10 Answers 0 Followers

Questions related from Lincoln Shane

What can you tell me about an E.coli strain named Lemo21 from NEB?

I am currently trying to express human tyrosinase in E.coli and as you know this enzyme being a membrane protein it comes with a fair share of difficulties relating to expression in BL21(DE3)....

04 October 2018 7,121 3 View

What are the chances that my protein is being degraded because my binding and washing buffers have no protease inhibitors?

I have been trying to express in BL21(DE3), purify and determine the Enzymatic kinetics of human Tyrosinase. After futile attempts at determining the kinetics of my purified protein ( expressed at...

07 May 2018 8,055 9 View

Can someone recommend a human Tyrosinase expression protocol?

Hello. I have been struggling with purification of human Tyrosinase expressed in E. Coli. In brief, I induce expression in E, Coli BL21 at an OD of 0.6 and then incubate further for 3 hours at 37...

07 May 2018 3,326 2 View

Protein purification protocol?

Hello fellow scientists, here i have a bit of a seemingly demoralizing issue with protein purification. Could you please help? I have attached a commassie image for clarity. 10mM imidazole, 50 mM...

26 December 2017 3,973 4 View

How does eBURST analyse and assign STs to clonal groups based on the ST numbers pasted into the program?

Hello everyone out there. I am doing an epidemiological study of clostridium difficile. I am using eBURST program to assign MLST STs to clonal groups or complexes. The program has assigned them to...

19 May 2016 847 0 View

Can anyone help with DNA extraction from skimmed milk C. difficile samples?

Hello fellow researchers, I am having a bit of an issue. I have been trying to extract DNA from skimmed milk clinical isolates in vain. The extraction is as follows: Add BHI plate cultured...

22 October 2015 8,722 6 View