My EMSA Blot is not sharp and blurry after exposure with a Phosphorimager (Typhoon). What can I do to fix that?

I'm currently trying to do some EMSA using P32. The first two gels looked perfect, with sharp bands and no background. However, all the following ones looked like the blot below. I'm not doing...

19 June 2024 1,943 2 View