Why can I not detect intracellular IL-17A in stimulated normal PBMCs?

Hello all, I am trying to stain for IL-17A in normal human PBMCs. I have used 3 different methods to stimulate my cells. 1) PHA (24 hours), 2) PMA/Ionomycin/Brefeldin A (5 hours), and 3) cell...

15 August 2016 5,836 3 View