Lily Oguh-Olayinka

3 Questions 2 Answers 0 Followers

Questions related from Lily Oguh-Olayinka

Has anyone observed large sediments after centrifugation cell lysates extracted in Laemmli buffer?

I treated some cancer cells with inhibitors, lysed them with laemmli buffer (containing protease and phosphatase inhibitors) by scraping on ice. They were left on the rotor for 16hrs in a cold...

05 June 2015 2,183 3 View

Does anyone know of a better to calculate drug synergy without calcusyn?

I am trying to combine 2 tyrosine kinase inhibitors and calculate synergy. The combination index I obtain are quite high (20, 60) for some of the drug combinations is this normal?

20 November 2014 6,162 13 View

Drugs dissolved in 100% DMSO formed crystals in RPMI - can anyone help?

I first dissolved the drug in 100% DMSO to 25mM and then further diluted in RPMI to 10mM and 1mM respectively. I realised that the 10mM concentration formed a lot of crystals and will not freeze....

20 June 2013 7,694 15 View