Why does the fluorescence intensity of Fluo-4 AM loaded cells decrease when observed under the fluorescence microscope?

I used a conventional fluorescence microscopy (mercury burner) to image the Calcium influx of a leukocyte. Firstly, the leukocyte were pre-loaded with Fluo-4 AM, and then treated with Thapsigargin...

17 July 2020 8,904 6 View