Lily Helen

2 Questions 6 Answers 0 Followers

Questions related from Lily Helen

Does anybody has experience on measuring ROS production of leukocyte anchored on BAM coated disk?

I am trying to test whether my stimuli could induce ROS production on leukocyte or not. I am using KG- cells. 1. Firstly, I coat the disk with BAM (Biocompatible anchor for membrane). 2. Then...

15 August 2020 6,755 4 View

Why does the fluorescence intensity of Fluo-4 AM loaded cells decrease when observed under the fluorescence microscope?

I used a conventional fluorescence microscopy (mercury burner) to image the Calcium influx of a leukocyte. Firstly, the leukocyte were pre-loaded with Fluo-4 AM, and then treated with Thapsigargin...

17 July 2020 8,934 6 View