Liliya N Kirpotina

3 Questions 6 Answers 0 Followers

Questions related from Liliya N Kirpotina

Is it possible specifically activate B-lymphocytes in the whole mice spleen culture? If yes, how to do that?

I'm trying to develop the functional assay for B-lymphocytes. I'm going to treat C57Bl/6 mice with compound of interest and immunotype cells in the whole blood and spleen by FAX (to compare with...

26 January 2017 2,133 4 View

Does anyone work with fixed human whole blood samples in order to stain CD11b with ICRF44 clone (FITC) for flow cytometry?

What is the best clone/company to use anti CD11b for FIXED whole blood samples? Do clones Bear1 or 94 work after fixation?

03 November 2014 7,310 3 View

What's the best primers for RT-PCR quantification of pro/antiinflammatory cytokines (TNF-alfa and so on) in human cells?

Usually we detect the cytokines production by ELISA. But sometimes it's not sensitive enough. So we decided to try to look at their genes expression as well. We are looking for their best primers....

01 January 1970 5,190 3 View