I am having trouble 'mating' (also called conjugation) a 70,000 bp DNA fragment from E. coli. into Streptomyces - can anyone help?

I am trying to transfer a 70,000bp secondary-metabolite-producing piece of DNA from E coli. 12567 cells into Stretpomyces Albus and Amycolatopsis Balhimycina by mating/conjugating them on an ISP4...

13 November 2013 5,580 1 View