Liangliang Sun

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Questions related from Liangliang Sun

iTRAQ data analysis

We recently acquired some iTRAQ based proteome quantitation data with Q-Exactive, but we have some problem for the data analysis. We firstly fractionated the sample with SCX column to 20...

01 January 2013 1,251 10 View

How to understand the difference of protein identification from immobilized trypsin and free trypsin digestion?

Usually free trypsin is used for protein digestion, followed by LC-MS identification. For complex sample, free trypsin digestion usually need at least several hours. Immobilized trypsin, trypsin...

08 August 2012 6,679 2 View