Is this a known technique: Replacing an homologous 2 Kb region of a plasmid by PCR using a mutation-containing 2 Kb ds-DNA fragment as a megaprimer?

I'm wondering if anyone is aware of published literature that describes the PCR cloning technique I've just used. I'm aware of this paper, which is very similar but introduces a DNA fragment as an...

01 January 2019 3,166 3 View