93kDa Fusion Protein Requires High pH to Elute of Column; oil in water emulsion?

I inherited a poorly working method for a 93kDa protein that needs significant revision.  Column is a waters XBridge BEH C4 300A and mobile phase is 0.1% triethylamine in water (A) and ACN (B);...

31 January 2017 6,888 4 View