Does anyone successfully generate stable cell line with 4T1 mouse cancer cell under Zeocin selection?

In my previous experiment, I used 4T1 cells to develop stable cell line. The vector I used is pBudCE4.1, Zeocin (500ug/mL) as the selection maker. The size of entire plasmid is 8.2kb. When...

08 August 2016 7,404 6 View