Li Li

5 Questions 8 Answers 0 Followers

Questions related from Li Li

What are the basal levels of TNF-alpha in RAW264.7 and J774 cell lines?

Does anyone use RAW264.7 or J774 to check the proinflammatory responses of certain stimulus? How much TNF-alpha is typically produced by resting cells for each cell line (~105 cells)?

10 October 2014 8,376 6 View

Does anyone know how to avoid having clumpy endothelial cell culture or break the clumps?

I need to run flow cytometry with endothelial cells. Maybe my cell culture is a bit over confluent. After detached, the cells with trypsin-EDTA are pipetted up and down so many times, and I still...

06 June 2014 721 11 View

Will Brefeldin A treatment affect the expression of surface protein if I need to detect two protein at the same time?

I need to run flow to detect the changes on protein expression after the stimulation of endothelial cell line with vesicles. There are two proteins I need to test, one secreted protein and one...

06 June 2014 648 2 View

Does anyone know how to calculate the Ct values from raw fluorescence data?

I know usually the software does the calculation. However, I run into a problem with one of my tests. I had raw data, melt curve and PCR products (checked by gel). But, the amplification plot was...

05 May 2014 9,425 3 View

How to prevent TB culture from clumping?

We are currently having problems with our TB cultures. They are very clumpy. Does anyone have suggestions on how to solve this problem?

11 November 2012 2,806 10 View