I'm isolating the compounds from hexane extract by sephadex lh20 column, how to find a better solvent system?

I think most of my compounds are non-polar, I tried solvent system 50dcm:50meoh, cannot separate at all, then add more hexane, using 70dcm:20hex:10meoh, better than before, but I don't know why,...

11 November 2024 2,118 3 View