How can I purify a recombinant H 10 tagged N protein from a co-contaminant post IMAC SEC?

Recombinant nucleocapsid protein expressed in E. coli with N terminal His10 tag (expected size 45 kDa ). Protein purified on IMAC crude FF washed with 250 mM imidazole/500 mM NaCl (pH 7.4) – not a...

10 May 2016 6,862 4 View