3 Questions 13 Answers 0 Followers
Questions related from Leontien Bosch
Update: Cells are growing fine in our lab now! I'm the only person in my lab starting on iPSc culture (we received a vial from another lab and all I have is some the medium they use for culture)...
03 August 2015 9,565 16 View
I'm trying to get in vitro transcribed RNA from a linearized plasmid, using the T7 MEGAscript kit. After the reaction I run a few ul on a 1% gel to check my RNA, but sometimes I get no RNA product...
11 December 2014 7,062 3 View
I started culturing Calu3 cells recently and I'm wondering if it is normal for this cell line that there's quite a lot of cell death (or at least, floating cells which I assume are dead cells)....
19 November 2014 5,862 6 View
