Anyone already ran a agarose gel with cDNA and found no bands or smear? (But in qPCR with housekeeping gene you have amplification and single melting?

Guys,I made a cDNA with High capacity kit and ran an agarose gel (1%, 1ul of cDNA) to check, BUT...there was nothing!!! I didn't had a single band, and not a smear either! Anyone have any idea...

08 August 2016 3,481 4 View