Leonardo Lopes-Luz

2 Questions 1 Answers 0 Followers

Questions related from Leonardo Lopes-Luz

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

11 March 2020 8,842 2 View

Does anyone already work with Protease Inhibitor Cocktail I (20-201 Merck / Sigma)?

I need to lyse insect cells (Tn5B lineage) to produce antigens using recombinant baculovirus. I need to use a protease inhibitor to protect my antigen. A researcher suggested that I use Halt ™...

01 January 1970 1,951 3 View