Leon Islas-Weinstein

3 Questions 9 Answers 0 Followers

Questions related from Leon Islas-Weinstein

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

04 May 2020 1,467 2 View

Can anyone recommend an RNA extraction protocol for complete mouse lungs (>100 mg of weight) using silical-gel columns?

In my lab, mice infected with tuberculosis develop regional inflammation in the first stages of disease. Therefore, to avoid unrepresented sampling (too much or too little inflamation), we want to...

06 August 2018 675 2 View

What is the time limit to place a whole brain sample in liquid nitrogen after cervical dislocation has taken place, for appropriate RNA extraction?

Besides obtaining the brain for RNA extraction, I need to obtain other organs in the mouse, such as the spleen, to measure neurotransmitters using HPLC (neurotransmitters degrade very rapidly!)....

27 December 2017 1,390 2 View