Does anyone have a protocol to prepare liposomes containing 100mM 5(6)-carboxyfluorescein?

I have been unable to solubilize the fluorophore at 100mM, even after plenty of heating and sonication.  I have also tried using calcein without success.  I am shooting for a 300mOsm HEPES...

06 May 2016 1,321 6 View