Lei Zhou

4 Questions 7 Answers 0 Followers

Questions related from Lei Zhou

Does anyone have experience with hela cells look smooth after media change?

Hela cells are cultured with DMEM+10%FBS+antibiotics in our lab using nunc 25-TC bottle. However, cells look awful (as attachment). But when Hela cells was plated in nunc 24-well plate and...

10 October 2013 7,782 7 View

Transfection efficiency of HeLa with PEI is low?

I am using PEI (polysciences, cat 23966-2) to transfect HeLa. I tried Plasmid:PEI 1:2,1:3 and 1:4. however, the transfection efficiency is about 5%. There is a protocol on the website which uses...

08 August 2013 1,310 1 View

Peritoneal macrophages floating after a period of time in culture?

I separate peritoneal macrophages following the traditional method. That is, after 1h adherence, cells are washed several times, and the adherent cells are deemed as macrophages. Here is the...

05 May 2013 9,332 20 View

Struggle with the HeLa culture.

The Hela cell was a gift from other lab, and it grows well in the other lab. However, after several passages, the cell look like the following pic. The media I am using is DMEM+L-...

03 March 2013 8,474 7 View