I need a protocol for PI staining using Ethanol as a fixative. Online protocols are to do with kits and I have only PI powder. Can anyone share?

I need to do fluorescent microscopy using Propidium Iodide. I initially fixed my cells with 4% paraformaldehyde and saw red stain in Control cells. It turns out PFA is cell permeable. So if anyone...

28 February 2024 421 3 View