Lee Roth

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Questions related from Lee Roth

Anyone used live imaging to trace cell cycle, especially mitotic stages? should I use a fluorophore or just phase imaging?

I have some strange phenotype somewhere between G2/M/G0 and I want to use live imaging to understand the time-line of the cell cycle in my treated cells, to understand if my cells are slow or...

09 September 2014 2,125 8 View

Which centrifuge and tubes can I use for 200ul @ 100,000g 37C 1h? Has anyone ever worked with the G-actin/F-actin separation kit of Cytoskeleton.com?

This cent. stage is for the separation of liquids from pellet. I followed the protocol of the kit: my sorvall tubes (2ml size) do not have a cap so after 1h @ 37C everything was evaporated.There...

05 August 2014 6,588 14 View