Lee Fuller

6 Questions 4 Answers 0 Followers

Questions related from Lee Fuller

Anyone willing to share Babesia vogeli?

I would be glad to cover the shipping to California via Fedex. Just some cold packs would work fine. I received some other canine species after 4 days shipping on cold packs (no longer cold at...

05 June 2023 2,095 0 View

How to match genes from one organism with another different kind of organism (database search)?

We believe that 4 proteins found in a piroplasmid are also found in a Dermacentor tick. How do you search for this kind of match in the databases?

26 May 2023 9,158 0 View

I found several proteins common to many ticks and one single piroplasmid. How do you search without the sequences?

These proteins are detectable by immunogical means and an be found on western blot lining up with each other. We have minute amounts, however, and no sequences yet.

30 April 2023 6,220 0 View

How to recreate 35 year-old creation of juvenile monocyte phenotype that grows Erlichia?

Long ago I put juvenile beagle monocytes into culture for use in propagating Ehrlichia canis, which had just been done by Ristic and Holland at U. of Illinois. I was using the host dog's serum at...

16 November 2022 4,103 0 View

How do we increase the percentage of secreting hybridomas (IgM antibody)?

We have 8 hyridomas that together form an oligoclonal mix to bind a particular protein in co-culture. The percentage of secretors has dropped over time and we've tried magnetic beads to collect...

11 November 2020 7,792 4 View

Help with separating human erythrocytes from mouse hybridomas?

We got separation of the monocyte/macrophage population using ficoll, but the mouse hybridomas are still mixed with the human erythrocytes. We've tried magnetics beads for selecting the hybridoma...

12 April 2020 9,948 4 View