Leah M Thornton Hampton

3 Questions 5 Answers 0 Followers

Questions related from Leah M Thornton Hampton

"Blotchy"/Incomplete fluorescent labeling in thyroid follicles of larval fish?

I have been successfully labeling thyroxine in the thyroid follicles of whole larval fish using a primary antibody specific for T4 (1:500) and AlexaFluor 488 as a secondary antibody (1:150). I...

28 May 2019 995 4 View

What is the purpose of exogenous superoxide dismutase in the nitroblue tetrazolium colorimetric assay?

I am currently using the classic colorimetric NBT assay to measure intracellular superoxide production in primary cell cultures. Cells are stimulated with PMA, NBT is reduced by superoxide to...

10 August 2018 5,038 2 View

Cells isolated from fish spleen dying after 24 hours of incubation?

I am VERY new to cell culture and am having some trouble keeping cells isolated from fathead minnow spleens alive for more than 24 hours or so. I'm attempting to replicate a lymphocyte...

24 June 2018 1,464 5 View