Leah M Thornton Hampton

2 Questions 3 Answers 0 Followers

Questions related from Leah M Thornton Hampton

"Blotchy"/Incomplete fluorescent labeling in thyroid follicles of larval fish?

I have been successfully labeling thyroxine in the thyroid follicles of whole larval fish using a primary antibody specific for T4 (1:500) and AlexaFluor 488 as a secondary antibody (1:150). I...

28 May 2019 983 4 View

What is the purpose of exogenous superoxide dismutase in the nitroblue tetrazolium colorimetric assay?

I am currently using the classic colorimetric NBT assay to measure intracellular superoxide production in primary cell cultures. Cells are stimulated with PMA, NBT is reduced by superoxide to...

10 August 2018 5,027 2 View