How to transfect primary macrophages (BMDM) with lipofectamine+siRNA without A) killing all cells before 48h, B) activating the macrophages?

1. Transfection: I need a double knockdown protocol because breeding is too slow. 2. Lipofectamine: I used RNAiMAX. 3. Cell density: I used 6-well plates, 160K cells per plate (the true macrophage...

03 December 2018 1,656 3 View