Leah Billingham

3 Questions 1 Answers 0 Followers

Questions related from Leah Billingham

CellROX detection via fluorescent plate reader?

Has anybody used a fluorescent plate reader to detect CellROX in adherent cells? Thanks!

17 March 2025 4,821 0 View

What centrifugation speed/time should I use for cultured primary microglia?

I've cultured primary microglia from neonates in 24-well plates, and typically I lift via scraping and put the cells straight into flow tubes for analysis. However, I would like to do additional...

17 March 2025 3,083 1 View

What's the best way to show RTPCR data when all the Ct values are high?

I am trying to figure out how to demonstrate that I cannot induce expression of a specific gene in a cell line. However, the gene is not expressed at baseline, so baseline Cts are very high. This...

05 July 2024 5,285 4 View