Leabaneng Tawe

3 Questions 4 Answers 0 Followers

Questions related from Leabaneng Tawe

Does anyone know what causes ghost bands in restriction enzyme digests?

I've carried out some restriction enzyme digests, using a restriction enzyme StyI for Duffy Antigen gene and I get a ghost bands as well as my expected product sizes. Any ideas on how to go about...

18 June 2018 5,197 2 View

DNA Extraction from Formalin Fixed Paraffin Embedded Tissue (FFPE) samples?

Hi, what is the best protocol to isolate DNA from FFPE tissue. I have tried KAPA Express Kit, NucliSENS Easymag, Organic Extraction, please advice further. Thank you!

23 August 2016 7,744 6 View

HPV DNA sequencing using gp5/6 primers?

Hello, I have DNA isolates which I used to performed PCR using MY011/09(~450bp outer) and gp5/6(~150bp nested) primers. The clean, specific PCR bands(~150bp) are then subjected to DNA...

01 January 1970 3,661 3 View