Le Phuong Nam

4 Questions 1 Answers 0 Followers

Questions related from Le Phuong Nam

Ligation into pBAC plasmid?

Good morning everyone. I would like to rescue the virus from clones. Now I have 4 inserts of DNA. DNA 1: ~4.5kb (digested by AscI/PacI) DNA 2: ~4.7kb (digested by PacI/KpnI) DNA 3: ~4.5kb...

4 weeks ago 2,368 1 View

Strategy of ligation?

Hi, I am wondering if it Is possible to follow this strategy. I had a plasmid A with my DNA 1 in AscI/StuI. I would like to insert my DNA 2 (both sides of the enzyme are StuI) into plasmid A at...

02 October 2024 3,200 3 View

How do I insert multiple large DNA into plasmid BAC?

Good morning everyone. I am working on cloning, and this is my first time doing that. I have trouble with cloning now. I have 4 inserts of DNA. DNA 1: ~2kb (digested by AscI/StuI) DNA 2: ~4.4kb...

06 August 2024 7,814 0 View

How do I insert large DNA into plasmid BAC system?

Good morning everyone. I am working on cloning; this is my first time doing that. I have trouble with cloning now. I have 4 inserts of DNA. DNA 1: ~2kb (digested by AscI/StuI) DNA 2: ~4.4kb...

01 January 1970 8,578 1 View